Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:20 |
Flow Cytometry (FCM): | 1:20 |
Western blot analysis of anti-HO-1 antibody (BM4010). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87-MG whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HO-1 antigen affinity purified monoclonal antibody (BM4010) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HO-1 at approximately 33 kDa. The expected band size for HO-1 is at 33 kDa.
IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (BM4010) .
HO-1/HMOX1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-HO-1/HMOX1 Antibody (BM4010) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of anti-HO-1 antibody (BM4010). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87-MG whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HO-1 antigen affinity purified monoclonal antibody (BM4010) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HO-1 at approximately 33 kDa. The expected band size for HO-1 is at 33 kDa.
IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (BM4010) .
HO-1/HMOX1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-HO-1/HMOX1 Antibody (BM4010) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.