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Anti-HO-1/HMOX1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

PB0050

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of anti-HO-1/HMOX1 antibody (PB0050). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat PC-12 whole cell lysates,
    Lane 2: mouse spleen tissue lysates,
    Lane 3: mouse RAW264.7 whole cell lysates,
    Lane 4: mouse NIH/3T3 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HO-1/HMOX1 antigen affinity purified polyclonal antibody (PB0050) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HO-1/HMOX1 at approximately 33 kDa. The expected band size for HO-1/HMOX1 is at 33 kDa.

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  • Figure 2. IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB0050).
    HO-1/HMOX1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

    all(3)
  • Figure 3. IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB0050).
    HO-1/HMOX1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

    all(3)

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产品名称
Anti-HO-1/HMOX1 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
bK286B10; heme oxygenase (decycling) 1; Heme oxygenase 1; HMOX1; HO; HO 1; HO1; HO-1; HO-1/HMOX1; HSP32
产品类型
Polyclonal
检验物种
mouse,rat
应用范围
WB,IHC
基因名称
HMOX1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived mouse HMOX1 recombinant protein (Position: E2-T261). Mouse HMOX1 shares 82% and 93% amino acid (aa) sequences identity with human and rat HMOX1, respectively.
计算分子量
33 kDa
实际分子量
33 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.
Uniprot ID
P14901  
文献引用格式
HO-1/HMOX1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0050)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
heme oxygenase (decycling) 1
蛋白名全称
Heme oxygenase 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-HO-1/HMOX1 Antibody

    筛选器: All WB IHC

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