Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of anti-HO-1/HMOX1 antibody (PB0050). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HO-1/HMOX1 antigen affinity purified polyclonal antibody (PB0050) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HO-1/HMOX1 at approximately 33 kDa. The expected band size for HO-1/HMOX1 is at 33 kDa.
Figure 2. IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB0050).
HO-1/HMOX1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB0050).
HO-1/HMOX1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. Western blot analysis of anti-HO-1/HMOX1 antibody (PB0050). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HO-1/HMOX1 antigen affinity purified polyclonal antibody (PB0050) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HO-1/HMOX1 at approximately 33 kDa. The expected band size for HO-1/HMOX1 is at 33 kDa.
Figure 2. IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB0050).
HO-1/HMOX1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB0050).
HO-1/HMOX1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.