Western blot (WB): | 1:500-2000 |
Flow Cytometry (Fixed): | 1:50-200 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti- HO-1/HMOX1 antibody (A00253-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates.
Use rabbit anti- HO-1/HMOX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HO-1/HMOX1 at approximately 33KD. The expected band size for HO-1/HMOX1 is at 33KD.
Figure 2. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- HO-1/HMOX1 antibody (A00253-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates.
Use rabbit anti- HO-1/HMOX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HO-1/HMOX1 at approximately 33KD. The expected band size for HO-1/HMOX1 is at 33KD.
Figure 2. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).