Anti-VEGFR2/KDR Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A00901-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody (A00901-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR2/KDR antigen affinity purified polyclonal antibody (A00901-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGFR2/KDR at approximately 180-250 kDa. The expected band size for VEGFR2/KDR is at 152 kDa.
all(5)
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody (A00901-2).
VEGFR2/KDR was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody (A00901-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody (A00901-2).
VEGFR2/KDR was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody (A00901-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody (A00901-2).
VEGFR2/KDR was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody (A00901-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody (A00901-2).
VEGFR2/KDR was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody (A00901-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)

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产品简介>

产品名称

Anti-VEGFR2/KDR Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD309; Fetal liver kinase 1; FLK 1; FLK1; KDR; Kinase insert domain receptor; VEGF Receptor 2; VEGFR; VEGFR 2; VEGFR2

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

KDR

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human VEGF Receptor 2 recombinant protein (Position: A20-L242).

计算分子量

152 kDa

实际分子量

180-250 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells. Flk1 is the mouse homolog of KDR.

Uniprot ID

P35968

RRID

AB_3081281

文献引用格式

VEGFR2/KDR Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00901-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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