产品名称
Anti-VEGFR2/KDR Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
CD309; Fetal liver kinase 1; FLK 1; FLK1; KDR; Kinase insert domain receptor; VEGF Receptor 2; VEGFR; VEGFR 2; VEGFR2
免疫原
A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2.
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
保存条件
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
KDR(Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al.(1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor(VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1(Flk1) in mouse, is exclusively expressed in endothelial cells(Plate et al., 1993). Flk1 is the mouse homolog of KDR(Matthews et al., 1991).
文献引用格式
VEGFR2/KDR Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0472)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
kinase insert domain receptor(a type III receptor tyrosine kinase)
蛋白名全称
Vascular endothelial growth factor receptor 2
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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