Anti-VEGFR2/KDR Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0410

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody (PB0410). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR2/KDR antigen affinity purified polyclonal antibody (PB0410) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGFR2/KDR at approximately 180-250 kDa. The expected band size for VEGFR2/KDR is at 152 kDa.
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产品名称

Anti-VEGFR2/KDR Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD309; Fetal liver kinase 1; FLK 1; FLK1; KDR; Kinase insert domain receptor; VEGF Receptor 2; VEGFR; VEGFR 2; VEGFR2

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

KDR

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human KDR different from the related mouse and rat sequences by three amino acids.

计算分子量

152 kDa

实际分子量

180-250 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al. (1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells (Plate et al., 1993). Flk1 is the mouse homolog of KDR (Matthews et al., 1991).

Uniprot ID

P35968

文献引用格式

VEGFR2/KDR Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0410)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

kinase insert domain receptor (a type III receptor tyrosine kinase)

蛋白名全称

Vascular endothelial growth factor receptor 2

实验方案>

实验步骤

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