Figure 1. Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse VEGFR2 Protein 1ng. anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for VEGF Receptor 2 at approximately 180-250KD. The expected band size for VEGF Receptor 2 is at 150KD.
all(7)
Figure 2. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of mouse kidney tissue. anti-VEGF Receptor 2 Antibody (A00901-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 3. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of rat kidney tissue. anti-VEGF Receptor 2 Antibody (A00901-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 4. ICC analysis of anti- KDR antibody (A00901-3).was detected in immunocytochemical section of NIH/3T3 cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog?#?BA1142) and counterstained with DAPI (blue)
all(7) 
Figure 5. Flow cytometry analysis of LLC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(7) 
Figure 6. Flow cytometry analysis of MFC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(7) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| ELISA(Cap): | 1:50-1:200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse VEGFR2 Protein 1ng. anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for VEGF Receptor 2 at approximately 180-250KD. The expected band size for VEGF Receptor 2 is at 150KD.
Figure 2. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of mouse kidney tissue. anti-VEGF Receptor 2 Antibody (A00901-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of rat kidney tissue. anti-VEGF Receptor 2 Antibody (A00901-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. ICC analysis of anti- KDR antibody (A00901-3).was detected in immunocytochemical section of NIH/3T3 cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog?#?BA1142) and counterstained with DAPI (blue)
Figure 5. Flow cytometry analysis of LLC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 6. Flow cytometry analysis of MFC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse VEGFR2 Protein 1ng. anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for VEGF Receptor 2 at approximately 180-250KD. The expected band size for VEGF Receptor 2 is at 150KD.
Figure 2. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of mouse kidney tissue. anti-VEGF Receptor 2 Antibody (A00901-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3).VEGF Receptor 2 was detected in paraffin-embedded section of rat kidney tissue. anti-VEGF Receptor 2 Antibody (A00901-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. ICC analysis of anti- KDR antibody (A00901-3).was detected in immunocytochemical section of NIH/3T3 cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog?#?BA1142) and counterstained with DAPI (blue)
Figure 5. Flow cytometry analysis of LLC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 6. Flow cytometry analysis of MFC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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