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Anti-VEGFR2/KDR Antibody (Clone#KDR-1)

Mouse monoclonal antibody

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筛选器: All WB IHC

BM0864

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-KDR antibody (BM0864).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: human HELA whole cell lysates. Use rabbit anti- KDR 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for KDR at approximately 190KD. The expected band size for KDR is at 152KD.

    all(2)
  • IHC analysis using anti- KDR antibody (BM0864). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(2)

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产品名称
Anti-VEGFR2/KDR Antibody (Clone#KDR-1)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD309; Fetal liver kinase 1; FLK 1; FLK1; KDR; Kinase insert domain receptor; VEGF Receptor 2; VEGFR; VEGFR 2; VEGFR2
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC
基因名称
KDR
克隆号
KDR-1
宿主
Mouse
抗体亚型
IgG1
免疫原
Recombinant human extracellular domain of VEGFR-2(KDR).
计算分子量
152 kDa
实际分子量
180-250 kDa
成分
200 ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The importance of VEGF and its receptor system in tumor growth and intervention in this system may provide promising approaches to cancer therapy. VEGF receptor 2 is a member of a receptor tyrosine kinase family. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues.
Uniprot ID
P35968  
文献引用格式
VEGFR2/KDR Antibody (Clone#KDR-1) (Boster Biological Technology, Wuhan, China. Catalog#BM0864)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
kinase insert domain receptor(a type III receptor tyrosine kinase)
蛋白名全称
Vascular endothelial growth factor receptor 2
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-VEGFR2/KDR Antibody (Clone#KDR-1)

    筛选器: All WB IHC

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