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Anti-Lamin A/C Antibody (Clone#22L53)

Rabbit monoclonal antibody

说明书

筛选器: All WB IHC ICC/IF FCM

M00438-5

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of anti-Lamin A/C antibody (M00438-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human PC-3 whole cell lysates,
    Lane 3: human HepG2 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin A/C antigen affinity purified monoclonal antibody (M00438-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lamin A/C at approximately 70-74 kDa. The expected band size for Lamin A/C is at 74 kDa.

    all(2)
  • Immunofluorescent analysis using the Antibody.

    all(2)
  • Immunofluorescent analysis using the Antibody.

    all(2)

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产品名称
Anti-Lamin A/C Antibody (Clone#22L53)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC; lamin A; lamin A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; Prelamin A/C; PRO1; progerin
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
LMNA
克隆号
22L53
抗体来源
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human Lamin A/C
计算分子量
74 kDa
实际分子量
70-74 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Uniprot ID
文献引用格式
Lamin A/C Antibody (Clone#22L53) (Boster Biological Technology, Wuhan, China. Catalog#M00438-5)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
Prelamin-A/C [Cleaved into: Lamin-A/C|70 kDa lamin|Renal carcinoma antigen NY-REN-32]
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
Flow Cytometry (FCM):1:50

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    Anti-Lamin A/C Antibody (Clone#22L53)

    筛选器: All WB IHC ICC/IF FCM

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