Anti-Lamin A/C Antibody

筛选器： All WB IHC IF FCM ICC/IF

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  Figure 1. Western blot analysis of anti- Lamin A/C antibody (PB9280). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: human PC-3 whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human HepG2 whole cell lysates.
  Use rabbit anti- Lamin A/C 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Lamin A/C at approximately 74KD, 63KD. The expected band size for Lamin A/C is at 74KD.

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  Figure 2. IHC analysis of Lamin A using anti-Lamin A antibody (PB9280).Lamin A was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lamin A Antibody (PB9280) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. IHC analysis of Lamin A using anti-Lamin A antibody (PB9280).Lamin A was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lamin A Antibody (PB9280) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  Figure 4. IHC analysis of Lamin A using anti-Lamin A antibody (PB9280).Lamin A was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lamin A Antibody (PB9280) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  Figure 5. IHC analysis of Lamin A using anti-Lamin A antibody (PB9280).
  Lamin A was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Lamin A Antibody (PB9280) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  Figure 6. IF analysis using anti- LMNA antibody (PB9280). detected in paraffin-embedded section of rat intestine tissues. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (greeen)(Catalog # BA1127) and counterstained with DAPI (blue).

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  Figure 9. Flow cytometry analysis of THP-1 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

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  Figure 7. ICC analysis of anti- LMNA antibody (PB9280).was detected in immunocytochemical section of A431 cells. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

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  Figure 8. ICC analysis of anti- LMNA antibody (PB9280).was detected in immunocytochemical section of NIH/3T3 cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).