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Anti-Lamin A Antibody (Clone#OTI3F6)

Mouse monoclonal antibody

筛选器: All WB ICC/IF

M00438-7

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Western blot analysis of anti-Lamin A antibody (M00438-7). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human SiHa whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Lamin A antigen affinity purified monoclonal antibody (M00438-7) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lamin A at approximately 74 kDa. The expected band size for Lamin A is at 74 kDa.

    all(2)
  • Figure 2. Immunofluorescent staining of Hela cells using anti-LMNA mouse monoclonal antibody or Isotype control (merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

    all(2)

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产品名称
Anti-Lamin A Antibody (Clone#OTI3F6)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC; lamin A; lamin A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; Prelamin A/C; PRO1; progerin
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, ICC/IF
基因名称
LMNA
克隆号
OTI3F6
抗体来源
Mouse
抗体亚型
IgG2b
免疫原
Full length human recombinant protein of human LMNA (NP_733821) produced in HEK293T cell.
计算分子量
74 kDa
实际分子量
74 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
Uniprot ID
文献引用格式
Lamin A Antibody (Clone#OTI3F6) (Boster Biological Technology, Wuhan, China. Catalog#M00438-7)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
Prelamin-A/C [Cleaved into: Lamin-A/C|70 kDa lamin|Renal carcinoma antigen NY-REN-32]
推荐稀释比
Western blot (WB):1:2000
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200

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    Anti-Lamin A Antibody (Clone#OTI3F6)

    筛选器: All WB ICC/IF

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