Figure 1. Western blot analysis of anti- Lamin A+C/LMNA antibody (M00438-6). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse small intestine tissue lysates.
Use mouse anti- Lamin A+C/LMNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Lamin A+C/LMNA at approximately 74KD. The expected band size for Lamin A+C/LMNA is at 70KD.
Figure 2. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
all(5) 
Figure 3. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human ovarian cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
all(5) 
Figure 4. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human bladder epithelial carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
all(5) 
Figure 5. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
all(5) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- Lamin A+C/LMNA antibody (M00438-6). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse small intestine tissue lysates.
Use mouse anti- Lamin A+C/LMNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Lamin A+C/LMNA at approximately 74KD. The expected band size for Lamin A+C/LMNA is at 70KD.
Figure 2. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 3. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human ovarian cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 4. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human bladder epithelial carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 5. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 1. Western blot analysis of anti- Lamin A+C/LMNA antibody (M00438-6). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse small intestine tissue lysates.
Use mouse anti- Lamin A+C/LMNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Lamin A+C/LMNA at approximately 74KD. The expected band size for Lamin A+C/LMNA is at 70KD.
Figure 2. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 3. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human ovarian cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 4. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human bladder epithelial carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 5. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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