Anti-Lamin A/C Antibody (Clone#5F3C12)

Mouse monoclonal antibody

说明书

筛选器： All WB IHC

M00438-6

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- Lamin A+C/LMNA antibody (M00438-6). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse small intestine tissue lysates.
Use mouse anti- Lamin A+C/LMNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Lamin A+C/LMNA at approximately 74KD. The expected band size for Lamin A+C/LMNA is at 70KD.
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Figure 2. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 3. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human ovarian cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 4. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human bladder epithelial carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 5. IHC analysis using anti- Lamin A+C/LMNA antibody (M00438-6). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Lamin A/C Antibody (Clone#5F3C12)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC; lamin A; lamin A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; Prelamin A/C; PRO1; progerin

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB,IHC

基因名称

LMNA

克隆号

5F3C12

抗体来源

Mouse

抗体亚型

IgG2b

免疫原

E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.

计算分子量

74 kDa

实际分子量

74 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Uniprot ID

P02545

文献引用格式

Lamin A/C Antibody (Clone#5F3C12) (Boster Biological Technology, Wuhan, China. Catalog#M00438-6)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

lamin A/C

蛋白名全称

Prelamin-A/C

实验方案>

实验步骤

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