Anti-TNF Alpha/TNFA Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of anti- TNFA/TNF Alpha antibody (BA0131). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human U-937 whole cell lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: mouse spleen tissue lysates.
  Use rabbit anti- TNFA/TNF Alpha 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TNFA/TNF Alpha at approximately 26KD. The expected band size for TNFA/TNF Alpha is at 26KD.

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  Figure 2. IHC analysis using anti- TNFA/TNF Alpha antibody (BA0131). detected in paraffin-embedded section of human B lymphocytic tumor tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.

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  Figure 3. Flow cytometry analysis of Caco-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB IHC FCM ELISA

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  Figure 2. IHC analysis using anti- YWHAG antibody (A04148-2). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 2. IHC analysis using anti- YWHAG antibody (A04148-2). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. Flow cytometry analysis of U87 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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Anti-GAPDH Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of anti- GAPDH antibody (A00227-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Caco-2 whole cell lysates,
  Lane 3: CCRF-CEM whole cell lysates,
  Lane 4: rat brain tissue lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: mouse brain tissue lysates,
  Lane 7: mouse liver tissue lysates.
  Use rabbit anti- GAPDH 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GAPDH at approximately 36KD. The expected band size for GAPDH is at 36KD.

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  Figure 2. IHC analysis using anti- GAPDH antibody (A00227-1).detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. IHC analysis using anti- GAPDH antibody (A00227-1).detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Anti-(Mouse) Claudin 6/CLDN6 Antibody (C-term)

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  Western blot analysis of lysate from mouse kidney tissue lysate, using Cldn6 Antibody (C-term). M08182-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

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  Overlay histogram showing colon26 cells stained with M08182-1 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08182-1, 1:25 dilution) for 60 min at 37oC. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Anti-(Mouse) IHH Antibody (N-term)

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  Anti-Ihh Antibody (N-term) at 1:2000 dilution + mouse kidney lysates
  Lysates/proteins at 20 μg per lane.
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
  Predicted band size : 45 kDa
  Blocking/Dilution buffer: 5% NFDM/TBST.

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  M01448 staining (Mouse) Ihh in mouse kidney sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37～C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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  Overlay histogram showing Jurkat cells stained with M01448 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (KRT12 Antibody, 1:25 dilution) for 60 min at 37oC. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Anti-14-3-3 Epsilon/YWHAE Antibody

筛选器： All WB IHC FCM ICC/IF ELISA

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  Figure 1. Western blot analysis of anti- YWHAE antibody (A01687-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human hepg2 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: human HEK293 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: human A549 whole cell lysates,Lane 8: human Raji whole cell lysates,Lane 9: Rat brain tissue lysates,Lane 10: Mouse brain tissue lysates,Lane 11: Mouse NIH/3T3 whole cell lysates,Lane 12: Mouse RAW264.7 whole cell lysates,Use rabbit anti- YWHAE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.

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  Figure 2. IHC analysis using anti- YWHAE antibody (A01687-4). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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  Figure 4. ICC analysis using anti- YWHAE antibody (A01687-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).

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Anti-14-3-3 Epsilon/YWHAE Antibody

筛选器： All WB IHC ICC/IF ELISA

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  Figure 1. Western blot analysis of anti- YWHAE antibody (A01687-5). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Jurkat whole cell lysates,
  Lane 3: HepG2 whole cell lysates,
  Lane 4: 293T whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse NIH/3T3 whole cell lysates.
  Use rabbit anti- YWHAE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAE at approximately 32KD. The expected band size for YWHAE is at 29KD.

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  Figure 2. IHC analysis using anti- YWHAE antibody (A01687-5). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. IHC analysis using anti- YWHAE antibody (A01687-5). detected in paraffin-embedded section of human prostate cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 4. IHC analysis using anti- YWHAE antibody (A01687-5). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 5. IHC analysis using anti- YWHAE antibody (A01687-5). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 6. IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-5).
  14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of A549 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB FCM ELISA

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  Figure 1. Western blot analysis of anti- YWHAG antibody (A04148-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: Mouse brain tissue lysates.Use rabbit anti- YWHAG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAG at approximately 28KD. The expected band size for YWHAG is at 28KD.

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  Figure 2. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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Anti-14-3-3 Sigma/SFN Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of anti-14-3-3 Sigma/SFN antibody (A01127). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human Hacat whole cell lysates,
  Lane 1: rat skin tissue lysates,
  Lane 2: mouse Hepa1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody (A01127) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Sigma/SFN at approximately 28 kDa. The expected band size for 14-3-3 Sigma/SFN is at 28 kDa.

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  Figure 2. IHC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (A01127). 14-3-3 sigma was detected in paraffin-embedded section of human rectal cancer tissues. anti-14-3-3 sigma Antibody (A01127) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127). 14-3-3 sigma was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  Figure 4. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127). 14-3-3 sigma was detected in paraffin-embedded section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-14-3-3 Sigma/SFN Antibody

筛选器： All WB IHC FCM ICC/IF

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  Figure 1. Western blot analysis of Anti-SFN antibody (BA3752). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: HELA whole cell lysates,Lane 2: PC-3 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates,Use rabbit Anti-SFN 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SFN at approximately 28KD. The expected band size for SFN is at 28KD.

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  Figure 2. IHC analysis using Anti-SFN antibody (BA3752) detected in paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. IHC analysis using Anti-SFN antibody (BA3752) detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 4. IHC analysis using Anti-SFN antibody (BA3752) detected in paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 7. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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  Figure 5. ICC analysis using anti- SFN antibody (BA3752). was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

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  Figure 6. ICC analysis using anti- SFN antibody (BA3752). was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).

Anti-14-3-3 Theta/YWHAQ Antibody

筛选器： All WB IHC ICC/IF FCM

Anti-2B4/CD244 Antibody

筛选器： All WB ELISA

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  Figure 1. Western blot analysis of Cd244 using anti-Cd244 antibody (A02527-2).
  Lane 1: rat spleen tissue lysates,
  Lane 2: rat spleen tissue lysates. anti-Cd244 antigen affinity purified polyclonal antibody (Catalog # A02527-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Cd244 at approximately 50KD. The expected band size for Cd244 is at 42KD.

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Anti-2B4/CD244 Antibody

筛选器： All WB ELISA

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  Figure 1. Western blot analysis of Cd244 using anti-Cd244 antibody (A02527-1).
  
  Lane 1: mouse spleen tissue lysates,
  Lane 2: mouse thymus tissue lysates,
  Lane 3: mouse RAW246.7 whole cell lysates,
  Lane 3: mouse SP20 whole cell lysates.
  anti-Cd244 antigen affinity purified polyclonal antibody (Catalog # A02527-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Cd244 at approximately 50KD. The expected band size for Cd244 is at 42KD.

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Anti-4EBP1/EIF4EBP1 Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of anti- EIF4EBP1 Antibody (A00968-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: HEL whole cell lysates,
  Lane 2: PC-3 whole cell lysates,
  Lane 3: HEK293 whole cell lysates,
  Lane 4: rat stomach tissue lysates,
  Lane 5: rat pancreas tissue lysates,
  Lane 6: mouse pancreas tissue lysates.
  Use rabbit anti- EIF4EBP1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for EIF4EBP1 at approximately 17KD. The expected band size for EIF4EBP1 is at 13KD.

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  Figure 2. IHC analysis using anti- EIF4EBP1 Antibody (A00968-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. Flow cytometry analysis of A431 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

Anti-5 Lipoxygenase/ALOX5 Antibody

筛选器： All IHC WB

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  IHC(P): Mouse Brain Tissue

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  IHC(P): Rat Brain Tissue

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  IHC(P): Human Intestinal Cancer Tissue

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  IHC(P): Human Mammary Cancer Tissue

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  All lanes: Anti-ALOX5 (PB0008) at 0.5ug/mlWB: SGC Whole Cell Lysate at 40ugPredicted bind size: 78KDObserved bind size: 78KD

Anti-5 Lipoxygenase/ALOX5 Antibody

筛选器： All IHC

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  Figure 1.IHC analysis using anti- ALOX5 antibody (BA1799-1). detected in paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Anti-5 Lipoxygenase/ALOX5 Antibody

筛选器： All WB IHC

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  Figure 1. Western blot analysis of Anti-ALOX5 antibody (BA2703). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: SW620 whole cell lysates,Lane 2: JURKAT whole cell lysates,Lane 3: COLO320 whole cell lysates,Lane 4: A549 whole cell lysates,Lane 5: MCF-7 whole cell lysates,Use rabbit Anti-ALOX5 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ALOX5 at approximately 78KD. The expected band size for ALOX5 is at 78KD.

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  Figure 2. IHC analysis using Anti-ALOX5 antibody (BA2703) detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Anti-5-HT-1F/HTR1F Antibody

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  Western Blot (WB) analysis of specific cells using SR-1F Polyclonal antibody.

Anti-5-HT-1F/HTR1F Antibody

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  Western blot analysis of extracts from COS-7 cells, using 5-HT-1F antibody A10463.